![]() ![]() Y the Y-coordinate in µm of the center of the feature-indicator associated with the feature, where (0,0) is the top left of the image.ĭia. the diameter in µm of the feature-indicator.į635 Median median feature pixel intensity at wavelength #1 (635 nm).į635 Mean mean feature pixel intensity at wavelength #1 (635 nm).į635 SD the standard deviation of the feature pixel intensity at wavelength #1 (635 nm).ī635 Median the median feature background intensity at wavelength #1 (635 nm).ī635 Mean the mean feature background intensity at wavelength #1 (635 nm).ī635 SD the standard deviation of the feature background intensity at wavelength #1 (635 nm). X the X-coordinate in µm of the center of the feature-indicator associated with the feature, where (0,0) is the top left of the image. ID the unique identifier of the feature derived from the Array List (up to 40 characters long, contained in quotation marks). Name the name of the feature derived from the Array List (up to 40 characters long, contained in quotation marks). The list below describes each column of data in the Results file. "Supplier=" Header field supplied in GAL file.ĭata record column headings Column titles for each measurement (see below). "Filters= " Emission filters used during acquisition (GenePix 4100 and 4200 only.) "LaserOnTime=5 5" The laser on-time for each laser, in minutes. "LaserPower=1 1" The power of each laser, in volts. "ScanPower=100 100" The amount of laser transmission during acquisition. "PMTGain=500 600" The PMT settings during acquisition. "Comment=hyb 2673" User-entered file comment. "LinesAveraged=1" The line average setting used to acquire the image. "Temperature=19.6127" The temperature of the scanner, in degrees C. "FocusPosition=0" The focus position setting used to acquire the image, in microns. "Scanner=GenePix 4000B " Type and serial number of scanner used to acquire the image. ![]() "Creator=GenePix 4.1.1.4" The version of the GenePix Pro software used to create the Results file. "JpegOrigin=390, 4320" The origin of the Results JPEG image (the bounding box of the analysis Blocks) relative to the scan area origin. "ImageOrigin=0, 0" The origin of the image relative to the scan area. "BackgroundSubtraction=LocalFeature" The background subtraction method selected in the Options settings. "Barcode=00331" The barcode symbols read from the image. "RatioFormulation=W1/W2 (635/532)" The ratio formulation of the ratio image, showing which image is numerator and which is denominator. "StdDev=Type 1" The type of standard deviation calculation selected in the Options settings. "JpegImage=C:\Genepix\demo.jpg" The name and path of the associated Jpeg image files. "NormalizationFactors=1 1" The normalization factor applied to each channel. "NormalizationMethod=None" The type of normalization method used, if applicable. "Wavelengths=635 532" Installed laser excitation sources in nm.Ĭ:\Genepix\Genepix.tif 1" The name and path of the associated TIF file(s). "PixelSize=10" Resolution of each pixel in µm. "GalFile=C:\Genepix\Demo.gal" The GenePix Array List file used to associate Names and IDs to each entry. "Settings=C:\Genepix\Genepix.gps" The name of the settings file that was used for analysis. "Type=GenePix Results 3" Type of ATF file. ![]() A sample GPR file header and a description of each entry are shown below:Ģ9 48 Number of optional header records and ![]()
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